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What is vivo bioluminescence imaging?

In vivo bioluminescent imaging (BLI) is a versatile and sensitive tool that is based on detection of light emission from cells or tissues. Bioluminescence has been used to track tumor cells, bacterial and viral infections, gene expression, and treatment response.

What is ICG fluorescence?

ICG is a tricarbocyanine dye which fluoresces, i.e. emits light, after excitation under near-infrared light at 806 nm light. ICG is highly soluble in water and binds to β-lipoproteins, particularly to albumin. Because of the high protein content of lymph, ICG accumulates in the lymphatic pathways and lymph nodes.

How does bioluminescent imaging work?

Bioluminescence imaging (BLI) is a technology that uses light emitted by enzyme-catalyzed reactions to report activity at the molecular level. Bioluminescent reporters require a small chemical substrate for non-invasive imaging in cell biology and small animal studies.

How does IVIS imaging work?

IVIS acquires a photographic image of the animal under white light and a quantitative bioluminescent or fluorescent signal, which is overlaid on the image. Photon flux from the tumor is proportional to the number of live cells expressing luciferase so bioluminescence correlates directly with tumor size.

Can luciferase be scanned?

The bioluminescence emitted can be detected and amplified using specialized cameras with highly sensitive detection systems [18], revealing the sites and levels of luciferase expression and activity from within a living animal.

What wavelength is bioluminescence?

Bioluminescent light is emitted in wavelengths between 400 and 720 nm, from violet into the near-infrared. The majority of bioluminescent marine organisms emit blue light (410–550 nm), which correlates with the peak sensitivities of the opsins of many marine organisms.

What is ICG made of?

ICG binds tightly to plasma proteins and becomes confined to the vascular system. ICG has a half-life of 150 to 180 seconds and is removed from circulation exclusively by the liver to bile juice….Indocyanine green.

What is ICG procedure?

Indocyanine Green Angiography (ICG) is a diagnostic procedure that uses ICG dye to examine the blood flow in the CHOROID – the layer of blood vessels which lies underneath the retina. Indocyanine Green dye is injected into a vein in the arm/hand.

What is bioluminescence tomography?

Bioluminescence tomography (BLT), as applied in preclinical molecular imaging, has attracted widespread attentions over biological and medical researches. With the coding genes for bioluminescent protein, it provides a specific tool to study the biological processes in vivo at the cellular and molecular levels [1–3].

What does IVIS mean?

IVIS

How do you test for bioluminescence?

How does in vivo bioluminescence imaging ( BLI ) work?

In vivo bioluminescent imaging (BLI) is a versatile and sensitive tool that is based on detection of light emission from cells or tissues. Bioluminescence, the biochemical generation of light by a living organism, is a naturally occurring phenomenon. Luciferase enzymes, such as that from the North A …

How much ICG can be detected by Phantom?

Phantom studies showed that signals at 4 mm depth are detected down to 3.3 ng ICG by PAT and 33 ng by FMT, with a nominal spatial resolution below 0.5 mm in PAT and limited to 1 mm in FMT.

How is bioluminescence used to image living organisms?

Luciferase enzymes, such as that from the North American firefly (Photinus pyralis), catalyze the oxidation of a substrate (luciferin), and photons of light are a product of the reaction. Optical imaging by bioluminescence allows a low-cost, noninvasive, and real-time analysis of disease processes at the molecular level in living organisms.